The Exchange has received revised Disclosure under Regulation 31(1) and 31(2) of SEBI (Substantial Acquisition of Shares & Takeovers) Regulations, 2011 on July 20, 2026 for Themis Medicare Ltd

TML/BSE/NSE/2026-27/15 20th July, 2026 Corporate Relationship Department Listing Department BSE Limited National Stock Exchange of India Limited Floor 25, Phiroze Jeejeebhoy Towers Exchange Plaza, Dalal Street, Mumbai- 400001 Bandra Kurla Complex, Scrip Code – 530199 Bandra (East), Mumbai- 400051 Symbol: THEMISMED Gujarat Themis Biosyn Limited Plot No. 69-C, G.I.D.C., Industrial Estate, Vapi, Valsad, Gujarat – 396195. Email: gtblmumbai@gtbl.in Dear Sir / Madam, Sub: Disclosure under Regulation 31 of the Securities and Exchange Board of India (Substantial Acquisition of Shares and Takeovers) Regulations, 2011. With reference to the captioned subject, we attach herewith the revised declaration in the prescribed format under Regulation 31 of SEBI (Substantial Acquisition of Shares and Takeovers) Regulations, 2011 and other applicable laws in respect of pledge release of 16,50,000 equity shares of Gujarat Themis Biosyn Limited on 8th July, 2026. This is for your information and record please. Thanking you, Yours faithfully, For Themis Medicare Limited Dr. Sachin D. Patel Managing Director & CEO DIN: 00033353 Themis Medicare Limited Corporate Office: 11/12 Udyog Nagar, S V Road, Goregaon (W), Mumbai – 400 104, India *Tel.: 91-22-67607080*Fax: 91-22-67607070/ 28746621 Regd. Office: Plot No. 69-A, G.I.D.C., Industrial Estate, Vapi-Gujarat CIN No.: L24110GJ1969PLC001590 *Tel/ Fax No.: Regd. Off.: 0260-2431447/ 2430219 *E-mail: themis@themismedicare.com*Website: www.themismedicare.com Format for disclosure by the Promoter(s) to the stock exchanges and to the Target Company for encumbrance of shares / invocation of encumbrance/ release of encumbrance, in terms of Regulation 31(1) and 31(2) of SEBI (Substantial Acquisition of Shares and Takeovers) Regulations, 2011. Particulars Details Name of the Target Company (TC) Gujarat Themis Biosyn Limited Names of the Stock Exchanges where the shares of the Target Company are listed BSE Limited and National Stock Exchange of India Limited Date of reporting 16th July, 2026 & 20th July 2026 Name of the promoter or PAC on whose shares encumbrance has been created / released / invoked Themis Medicare Limited Name of Promoter holding Promoter Details of events perta ining to encumbrance Post-event holding of the in the target holding already encumbered shares – promoter( comp any encum bered Number {creation s) or PAC [(2)+(3)] / release [(2)-(3)] with him / invocation [(1)-(3)]} Number % of Number % of Type of Date of Type of Reasons for encumbrance Number % of Name of the Number % of total total event creation/ encumbrance share entity in total share share (creation / release/ (pledge/ lien/ capital whose favor share capital capit release / invocation of non disposal shares capital al invocation) encumbrance undertaking/ encumbered others) Themis 2,27,74, 16,50,00 Jio Credit Medicare 20.90 1.51% Release 08/07/2026 Pledge Repayment of Loan 16,50,000 1.51% NIL NIL 847 0 Limited Limited The names of all the promoters, their shareholding in the target company and their pledged shareholding are as on the reporting date. For Themis Medicare Limited Dr. Sachin D Patel Managing Director & CEO DIN: 00033353 Date: 20th July, 2026 Place: Mumbai