Enclosed herewith Resignation of M/s Vishal J Bhanushali and Associated with effect from 13th August 2026

Aspira Pathlab & Diagnostics Limited Regd. Office: Flat No. 2, R D Shah Building, Shraddhanand Road, Opp. Railway Station, Ghatkopar (W), Mumbai 400 086 Corporate Office: 6 & 7, Bhaveshwar Arcade, Near Shreyas Junction LBS Marg, Behind Saraswat Bank, Ghatkopar (W), Mumbai-400086 CIN: L85100MH1973PLC289209 Date: August 03, 2026 BSE Limited, Listing Department Phiroze Jeejeebhoy Towers, Dalal Street, Mumbai-400001. Security Code: 540788 Security ID: ASPIRA Sub: Resignation from the post of Internal Auditor Ref:Regulation 30 of SEBI (Listing Obligation & Disclosure Requirements) Regulations, 2015 Dear Sir/Madam, Pursuant to Regulation 30 of the SEBI (Listing Obligations and Disclosure Requirements) Regulations, 2015 (‘SEBI LODR’) we wish to inform you that M/s. Vishal J Bhanushali and Associates resigned from post of Internal Auditor with effect from 13.08.2026 due to preoccupation and other personal reasons. The details as required under Regulation 30 of Listing Regulations read with SEBI Master Circular No. HO/49/14/14(7)2025-CFD-POD2/I/3762/2026 dated 30th January 2026 are provided in Annexure-I. Kindly take the same on record. Thanking you. Yours Faithfully, For Aspira Pathlab & Diagnostics Limited Nikunj Mange Executive Director DIN: 08489442 INDIA’S FIRST 0227197 5756, 022 2513 9090 www.aspiradiagnostics.com FULLY INTEGRATED LAB support@aspiradiagnostics.com │info@aspiradiagnostics.com Aspira Pathlab & Diagnostics Limited Regd. Office: Flat No. 2, R D Shah Building, Shraddhanand Road, Opp. Railway Station, Ghatkopar (W), Mumbai 400 086 Corporate Office: 6 & 7, Bhaveshwar Arcade, Near Shreyas Junction LBS Marg, Behind Saraswat Bank, Ghatkopar (W), Mumbai-400086 CIN: L85100MH1973PLC289209 Annexure-1 Disclosure of information pursuant to Regulation 30 of Listing Regulations read with SEBI Master Circular No. HO/49/14/14(7)2025-CFD-POD2/I/3762/2026 dated 30th January 2026. Sr Particulars Disclosure 1. Reason for change viz. M/s.Vishal J Bhanushali and Associates resigned appointment, re-appointmfernomt, post of Internal Auditor due to preoccupation resignation, removal, and other personal reasons death or otherwise; 2. Date of cessation August 13, 2026 3. Brief Profile Not applicable 4. Disclosure of relationshipNso t applicable between Directors INDIA’S FIRST 0227197 5756, 022 2513 9090 www.aspiradiagnostics.com FULLY INTEGRATED LAB support@aspiradiagnostics.com │info@aspiradiagnostics.com VISHAL J. BHANUSHALI & ASSOCIATES CHARTERED ACCOUNTANTS Reg. Off: D-2202, Choice Ambe Sagar, Dr R P Road, Opp Gyansarita School, Mulund (W), Mumbai – 40008 To Date: 03/08/2026 The Board of Directors Aspira Pathlab & Diagnostics Limited Flat No. 2, R.D. Shah Building, Shraddhanand Road, Opp. Ghatkopar Railway Station, Ghatkopar (West), Mumbai City, Mumbai, Maharashtra – 400086. Subject: Resignation from Internal Audit Dear Directors, I, Vishal J Bhanushali Proprietor of M/s. Vishal Bhanushali & Associates, hereby tender my resignation from the position of Internal Auditor of Aspira Pathlab & Diagnostics Limited due to preoccupation and other personal reasons with effect from 13/08/2026. I express my sincere gratitude to the Board and the Management for the opportunity and support extended to me during my association with the Company. I request the Board to take the necessary steps in connection with my resignation, as required under applicable laws. Thanking You For Vishal J. Bhanushali & Associates Chartered Accountants Firm Reg. No.: 145806W Vishal J. Bhanushali Proprietor Membership No: 179979