Enclosed herewith Resignation of Mr. Pankaj Jashwant Shah from post of Managing Director and Chief Executive Officer with effect from 03.08.2026

Aspira Pathlab & Diagnostics Limited Regd. Office: Flat No. 2, R D Shah Building, Shraddhanand Road, Opp. Railway Station, Ghatkopar (W), Mumbai 400 086 Corporate Office: 6 & 7, Bhaveshwar Arcade, Near Shreyas Junction LBS Marg, Behind Saraswat Bank, Ghatkopar (W), Mumbai-400086 CIN: L85100MH1973PLC289209 Date: August 03, 2026 BSE Limited, Listing Department Phiroze Jeejeebhoy Towers, Dalal Street, Mumbai-400001. Security Code: 540788 Security ID: ASPIRA Sub: Resignation from the post of Managing Director and Chief Executive Director Ref:Regulation 30 of SEBI (Listing Obligation & Disclosure Requirements) Regulations, 2015 Dear Sir/Madam, Pursuant to Regulation 30 of the SEBI (Listing Obligations and Disclosure Requirements) Regulations, 2015 (‘SEBI LODR’) we wish to inform you that Mr. Pankaj Jashwant Shah (DIN: 02836324), has tendered his resignation as Managing Director and Chief Executive Director due to personal reasons with immediate Effect i.e. 03.08.2026. The details as required under Regulation 30 of Listing Regulations read with SEBI Master Circular No. HO/49/14/14(7)2025-CFD-POD2/I/3762/2026 dated 30th January 2026 are provided in Annexure-I. Kindly take the same on record. Thanking you. Yours Faithfully, For Aspira Pathlab & Diagnostics Limited Nikunj Mange Executive Director DIN: 08489442 INDIA’S FIRST 0227197 5756, 022 2513 9090 www.aspiradiagnostics.com FULLY INTEGRATED LAB support@aspiradiagnostics.com │info@aspiradiagnostics.com Aspira Pathlab & Diagnostics Limited Regd. Office: Flat No. 2, R D Shah Building, Shraddhanand Road, Opp. Railway Station, Ghatkopar (W), Mumbai 400 086 Corporate Office: 6 & 7, Bhaveshwar Arcade, Near Shreyas Junction LBS Marg, Behind Saraswat Bank, Ghatkopar (W), Mumbai-400086 CIN: L85100MH1973PLC289209 Annexure-1 Disclosure of information pursuant to Regulation 30 of Listing Regulations read with SEBI Master Circular No. HO/49/14/14(7)2025-CFD-POD2/I/3762/2026 dated 30th January 2026. Sr Particulars Disclosure 1. Reason for change viz. Mr. Pankaj Jashwant Shah, (DIN: appointment, re-appointment, 02836324) has tendered resignation, removal, her resignation from the death or otherwise; post of Managing Director and Chief Executive Director due to personal reasons. 2. Date of cessation August 03, 2026. 3. Brief Profile Not applicable 4. Disclosure of relationships Not applicable between Directors INDIA’S FIRST 0227197 5756, 022 2513 9090 www.aspiradiagnostics.com FULLY INTEGRATED LAB support@aspiradiagnostics.com │info@aspiradiagnostics.com