Certificate under Regulation 74(5) of the SEBI (Depositories and Participants) Regulations, 2018

Regd. Office: Plot No. 41/3 & 41/5, Village Lohop, Lohop Chowk Rd., Tal. Khalapur – 410202. Dist. Raigad, Maharashtra. CIN: L24115MH1988PLC048126 Email: tulaseebio@gmail.com Date: October 14 2026 The Corporate Relationship Department Bombay Stock Exchange Limited 25th Floor, P J Tower, Dalal Street Fort, Mumbai – 400 001 Scrip Code: 524514 Sub: Certificate under Regulation 74(5) of the SEBI (Depositories and Participants) Regulations, 2018 for the quarter and half year ended September 30, 2026: Dear Sir/Madam, Pursuant to Regulation 74 (5) of the SEBI (Depositories and Participants) Regulations, 2018, for the quarter and half year ended September 30, 2026 based on the certificate received from M/s MUFG Intime India Pvt. Ltd (RTA), which is enclosed herewith, certifying compliance with Regulation 74(5) of the SEBI (Depositories and Participants) Regulations, 2018. Request you to kindly take the same on your record. For TULASEE BIO-ETHANOL LIMITED Mr. Kapil Nagpal Managing Director DIN: 01929335 MUFG Intime India Private Limited (Formerly Link Intime India Private Limited) CIN: U67190MH1999PTC118368 Registered Address: C-101, Embassy 247, L.B.S. Marg, Vikhroli (West), Mumbai - 400083. Tel: +91 22 4918 6000 www.in.mpms.mufg.com Date : 03-10-2026 The Compliance Officer/ Company Secretary TULASEE BIO ETHANOL LTD PLOT NO 4/3 & 4/5 LOHOP CHOWK ROAD VILLAGE LOHOP TALUK KHALAPUR 410202 RAIGARH MH PINCODE : 410206 Sub : Confirmation Certificate under Regulation 74(5) of Securities and Exchange Board of India (Depositories and Participants) Regulations, 2018. Dear Sir/Madam, In reference to the above captioned regulation, we hereby confirm that the securities received from the depository participants for dematerialisation during the quarter ended 30th September 2026, were confirmed (accepted/rejected) to the depositories by us and that securities comprised in the said certificates have been listed on the stock exchanges where the earlier issued securities are listed. We hereby also confirm that the security certificates received for dematerialisation have been confirmed/rejected and the security certificates received were mutilated and cancelled after due verification by the depository participant and the name of the depositories have been substituted in the register of members as the registered owner within the prescribed timelines. We request you to kindly take note of the above in your records. Thanking you, Yours faithfully, For MUFG Intime India Private Limited (Formerly Link Intime India Private Limited) Ashok Shetty Sr. Vice President-Corporate Registry MUFG Intime India Private Limited A part of MUFG Corporate Markets, a division of MUFG Pension & Market Services