Certificate under SEBI (Depositories and Participants) Regulations, 2018

GUJARAT THEMIS BIOSYN LIMITED CIN: L24230GJ1981PLC004878 REGD. OFFICE &FACTORY: 69/C GIDC INDUSTRIAL ESTATE, VAPI – 396 195, DIST. VALSAD, GUJARAT, INDIA TEL: 0260-2430027 / 2400639 E-mail:hrm@gtbl.in.net GTBL/BSE/NSE/2026-27/69 12th October, 2026 Listing Department Department of Corporate Services National Stock Exchange of India Limited BSE Limited Exchange Plaza, C-1, Block-G Phiroze Jeejeebhoy Towers, Dalal Street Bandra Kurla Complex Mumbai 400001 Bandra East, Mumbai 400051 Symbol: GUJTHEM Scrip Code: 506879 Dear Sir / Madam, Sub: Certificate under Regulation 74(5) of SEBI (Depositories and Participants) Regulations, 2018 for the quarter ended 30th September, 2026. We are enclosing herewith a Certificate under Regulation 74(5) of SEBI (Depositories and Participants) Regulations, 2018 for the quarter ended 30th September, 2026, received from MUFG lntime India Pvt. Ltd., Registrar and Share Transfer Agent of our Company. This is for your information and record. Thanking you, Yours faithfully, For Gujarat Themis Biosyn Limited Vineet Gawankar Company Secretary and Compliance Officer MUMBAI OFFICE: Themis House, 11/12 Udyog Nagar, S.V Road, Goregaon (West), Mumbai – 400 104 Tel: 91-22-67607080 / 28757836 Fax: 28746621 / 67607019; E-mail: gtblmumbai@gtbl.in Website Address: www.gtbl.in MUFG Intime India Private Limited (Formerly Link Intime India Private Limited) CIN: U67190MH1999PTC118368 Registered Address: C-101, Embassy 247, L.B.S. Marg, Vikhroli (West), Mumbai - 400083. Tel: +91 22 4918 6000 www.in.mpms.mufg.com Date : 03-10-2026 The Compliance Officer/ Company Secretary GUJARAT THEMIS BIOSYN LIMITED THEMIS HOUSE, 11/12 UDYOG NAGAR S.V ROAD, POST BOX NO. 17529 GOREGAON (WEST), MUMBAI - 400104 MUMBAI PINCODE : 400104 Sub : Confirmation Certificate under Regulation 74(5) of Securities and Exchange Board of India (Depositories and Participants) Regulations, 2018. Dear Sir/Madam, In reference to the above captioned regulation, we hereby confirm that the securities received from the depository participants for dematerialisation during the quarter ended 30th September 2026, were confirmed (accepted/rejected) to the depositories by us and that securities comprised in the said certificates have been listed on the stock exchanges where the earlier issued securities are listed. We hereby also confirm that the security certificates received for dematerialisation have been confirmed/rejected and the security certificates received were mutilated and cancelled after due verification by the depository participant and the name of the depositories have been substituted in the register of members as the registered owner within the prescribed timelines. We request you to kindly take note of the above in your records. Thanking you, Yours faithfully, For MUFG Intime India Private Limited (Formerly Link Intime India Private Limited) Ashok Shetty Sr. Vice President-Corporate Registry MUFG Intime India Private Limited A part of MUFG Corporate Markets, a division of MUFG Pension & Market Services