Closure of Trading Window for quarter ended September 30,2026

Aspira Pathlab & Diagnostics Limited Registered. Office: Flat No. 2, R D Shah Building, Shraddhanand Road, Opp. Railway Station, Ghatkopar (W), Mumbai-400086 Corporate Office: 6 & 7, Bhaveshwar Arcade, Near Shreyas Junction LBS Marg, Behind Saraswat Bank, Ghatkopar (W), Mumbai-400086 CIN: L85100MH1973PLC289209 Date: September 23, 2026 Listing Department, BSE Limited, Phiroze Jeejeebhoy Towers, Dalal Street, Mumbai-400001. Scrip Code: 540788 Security ID: ASPIRA Sub: Closure of Trading Window Ref: SEBI (Prohibition of Insider Trading) Regulations, 2015 This is to inform that, pursuant to the SEBI (Prohibition of Insider Trading) Regulations, 2015 (as amended) and the Company’s Code of Conduct for Prevention of Insider Trading, the Trading Window for dealing in the securities of the Company shall remain closed for Designated Persons and their immediate relatives w.e.f October 01, 2026 till the declaration of Financial Results for the half year ended September 30, 2026 and 48 (Forty-eight) hours thereafter. The date of the Board Meeting for declaration of the Financial Results for the half year ended September 30, 2026 shall be intimated in the due course. You are requested to kindly take the above on you records. Thanking you. Yours sincerely. For Aspira Pathlab & Diagnostics Limited Neetu Maurya Company Secretary & Compliance Officer INDIA’S FIRST 0227197 5756, 022 2513 9090 www.aspiradiagnostics.com FULLY INTEGRATED LAB support@aspiradiagnostics.com │info@aspiradiagnostics.com